Target Enabling Packages (TEPs)
Jola Kopec, Elzbieta Rembeza, Mark McLaughlin, Oleg Fedorov, Claire Strain-Damerell, Solenne Goubin, Sabrina MacKinnon, Nicola Burgess-Brown, Paul Brennan, Alex MacKenzie, Paulo Arruda, Wyatt Yue. A Target Enabling Package, 2017, Volume 10. This work provides the early tools to develop substrate reduction inhibitors for a genetic childhood seizure disorder, with the hypothesis to target the enzyme (AASS) upstream of the defective gene (ALDH7A1) in the human lysine metabolic pathway. This TEP package includes recombinant human AASS purification protocols, structures of the AASS second domain in different states, in vitro assays to detect ligand binding (differential scanning fluorimetry) and enzyme activity (NADH formation) of human AASS, as well as initial chemical matters.